Establishment of cell lines for quality control of prenatal genetic diagnosis by gene transfection / 浙江大学学报·医学版

ABSTRACT

OBJECTIVE@#To establish a cell lines for quality control of prenatal genetic diagnosis.@*METHODS@#The recombined SV40LTag-pcDNA3.1(-) vector was constructed and transfected by lipidosome into human amniotic fluid cells with common aneuploidy. Positive clones were screened by G418, and the immortality of transfected cell line was identified.@*RESULTS@#Cell line with karyotype of 46, XY, t(8;19)(q24.3;q13.1) from primary amniotic fluid cells was established. Karyotype analytical results indicated that the cell line at its 15th generation maintained the same karyotype of its primary cell.@*CONCLUSIONS@#Gene can lead to immortality of amniotic fluid cells, which contributes to preparing cell lines for internal and external quality control in prenatal genetic diagnosis.