Protective effect of curcumin on dopamine neurons in Parkinson's disease and its mechanism / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 480-486, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-775290
ABSTRACT
OBJECTIVE@#To investigate the effect of curcumin on dopamine neurons in Parkinson's disease (PD) and its mechanism.@*METHODS@#SH-SY5Y human neuroblastoma cells were treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to establish the PD cell model. The model cells were treated with curcumin and/or autophagy inhibitor 3-MA. After 48 h of drug treatment, the number of surviving dopamine neurons was detected by tyrosine hydroxylase immunofluorescence method. Western blotting was used to detect protein expression of α-Synuclein (α-Syn), transcription factor EB (TFEB) and autophagy-related proteins lysosome-associated membrane protein 2A (LAMP2A) and microtubule-associated protein 1 light chain 3-Ⅱ(LC3-Ⅱ); RT-PCR was used to detect mRNA expression of α-Syn.@*RESULTS@#Compared with MPTP model group, curcumin increased the number of surviving dopamine neurons(<0.01), decreased both protein expression and mRNA expression of α-Syn (all <0.01), and increased protein expression of TFEB, LAMP2A and LC3-Ⅱ (all <0.01). When curcumin and 3-MA were given concurrently, the number of surviving dopamine neurons, protein expression of TFEB, LAMP2A and LC3-Ⅱ increased (<0.05 or <0.01), and both protein expression and mRNA expression of α-Syn decreased (<0.05 or <0.01) compared with MPTP model group; but the number of surviving dopamine neurons and protein expression of LAMP2A and LC3-Ⅱ decreased compared with curcumin group (all <0.05).@*CONCLUSIONS@#Curcumin exerts protective effect on dopamine neurons in PD, which may be associated with enhancing autophagy and promoting the clearance of α-Syn.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parkinson Disease
/
Pharmacology
/
Cell Line
/
Curcumin
/
Alpha-Synuclein
/
Dopaminergic Neurons
/
Metabolism
/
Mice, Inbred C57BL
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2018
Type:
Article
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