Rapid analysis on chemical constituents in Qige Keli by RRLC-Q-TOF-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4240-4247, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-775352
ABSTRACT
The qualitative analysis method of RRLC-Q-TOF-MS/MS was established for determine the chemical constituents in Qige Keli. Kramosil C₁₈ column (4.6 mm×150 mm, 3.5 μm) was used at the temperature of 30 °C. The mobile phase was 0.2% formic acid and acetonitrile by gradient elution, with a flow rate at 1.0 mL·min⁻¹, and the injection volume was 10 μL. The high-resolution quadrupole time-flight mass spectrometry was used as detector with electrospray ion source in both positive and negative models. On the basis of medicinal materials, reference materials, literature reports, and mass spectrometry data, the chemical composition in the Qige Keli was identified. A total of 44 compounds were identified, including 3 flavonoids, 21 flavonoid glycosides, 8 organic acids, 6 lactones, and 3 saponins. The results laid the foundation for the quality control of Qige Keli and the further research on pharmacodynamic materials.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saponins
/
Acids
/
Flavonoids
/
Drugs, Chinese Herbal
/
Chemistry
/
Spectrometry, Mass, Electrospray Ionization
/
Tandem Mass Spectrometry
/
Phytochemicals
/
Glycosides
/
Lactones
Type of study:
Qualitative research
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2018
Type:
Article
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