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Protective effect of catalpolon destruction of tight junctions of high glucose induced BMECs / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4118-4124, 2018.
Article in Chinese | WPRIM | ID: wpr-775369
ABSTRACT
This paper aimed to observe the protective effect of catalpol on the high glucose induced destruction of tight junctions of rat primary brain microvascular endothelial cells (BMECs). Catalpol co-administrated with high glucose increased BMECs survival, decreased its ET-1 secretion, and improved transmembrane electrical resistance in a time-dependent manner. Furthermore, transmission electron microscopy was used to observe catalpol's protective effect on tight junction. Fluorescence staining displayed that catalpol reversed the rearrangement of the cytoskeleton protein F-actin and up-regulated the tight junction proteins claudin-5 and ZO-1, which were further demonstrated by the mRNA expression levels of claudin-5, occludin, ZO-1, ZO-2, ZO-3, -actintin, vinculin and cateinins. This study indicated that catalpol reverses the disaggregation of cytoskeleton actin in BMECs and up-regulates the expression of tight junction proteins, such as claudin-5, occludin, and ZO-1, and finally alleviates the increase in high glucose-induced BMECs injury.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phosphoproteins / Brain / Actin Cytoskeleton / Cells, Cultured / Actins / Tight Junctions / Cell Biology / Endothelial Cells / Iridoid Glucosides Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phosphoproteins / Brain / Actin Cytoskeleton / Cells, Cultured / Actins / Tight Junctions / Cell Biology / Endothelial Cells / Iridoid Glucosides Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2018 Type: Article