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Establishment of a quantitative real-time PCR for rapid detection of Panax quinquefolius / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4050-4054, 2018.
Article in Chinese | WPRIM | ID: wpr-775380
ABSTRACT
In this study, the specific primers and probes of Panax quinquefolius were designed for a quantitative real-time PCR, and the rapid identification method of P. quinquefolius was established by optimizing conditions. The method was used to validate 43 samples of the traditional Chinese medicine,and the results showed that 22 samples of P. quinquefolius were identified accurately. The limit of detection of the method can be reach to 1×10⁻⁴ ng. The method is accurate, fast, sensitive and specifically.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Reference Standards / Drugs, Chinese Herbal / DNA Probes / DNA Primers / Real-Time Polymerase Chain Reaction / Genetics / Panax Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Reference Standards / Drugs, Chinese Herbal / DNA Probes / DNA Primers / Real-Time Polymerase Chain Reaction / Genetics / Panax Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2018 Type: Article