Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 205-215, 2019.
Article
in English
| WPRIM
| ID: wpr-775425
ABSTRACT
The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Spectrum Analysis
/
Tyrosine 3-Monooxygenase
/
Bipolar Disorder
/
Image Processing, Computer-Assisted
/
Locus Coeruleus
/
Immunohistochemistry
/
Sensitivity and Specificity
/
Depressive Disorder, Major
/
Receptor, ErbB-4
Type of study:
Diagnostic study
Limits:
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
English
Journal:
Neuroscience Bulletin
Year:
2019
Type:
Article
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