Effects of Transcription Factor MZF-1 on Transcriptive Regulation of Acute Monocytic Leukemia-related Gene MLAA-34 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1463-1468, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-775698
ABSTRACT
OBJECTIVE@#To investigate the transcriptional regulation of transcription factor MZF-1 on acute monocytic leukemia-related gene MLAA-34.@*METHODS@#The effect of MZF-1 on the transcriptional activity of MLAA-34 gene promoter was analyzed by luciferase reporter gene detection system and site-directed mutation technique. The EMSA and ChIP assay were used to verify whether MZF-1 directly and specifically binds to the core region of MLAA-34 promoter. The over-expression vector and interference vector of MZF-1 were constructed to transfect U937 cells, and RT-PCR and Western blot were used to detect the transcription and expression changes of MLAA-34 gene.@*RESULTS@#The transcription factor MZF-1 had a regulatory effect on MLAA-34 gene expression, and the relative luciferase activity was decreased after MZF-1 binding point mutation (P<0.01). EMSA and ChIP experiments demonstrated that MZF-1 could directly bind to MLAA-34 promoter and play a regulatory role. In the over-expression test, the increase of MZF-1 could up-regulate the expression of MLAA-34 (P<0.05). In the interference test, the decrease of MZF-1 could down-regulate the expression of MLAA-34 (P<0.05).@*CONCLUSION@#Transcription factor MZF-1 can bind to the transcriptional regulatory region on the promoter of MLAA-34 gene and promote the transcription of MLAA-34 gene in acute monocytic leukemia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
Gene Expression Regulation, Neoplastic
/
Leukemia, Monocytic, Acute
/
Promoter Regions, Genetic
/
Genes, Reporter
/
Apoptosis Regulatory Proteins
/
Hepatocyte Nuclear Factor 1-alpha
/
Kruppel-Like Transcription Factors
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2019
Type:
Article
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