Role of Citrullinated Fibrinogen Peptides in the Activation of CD4 T Cells from Patients with Rheumatoid Arthritis
Immune Network
;
: 116-122, 2013.
Article
in English
| WPRIM
| ID: wpr-77570
ABSTRACT
This study was conducted to determine whether CD4 T cell responses to citrullinated fibrinogen occur in patients with rheumatoid arthritis (RA), especially in HLA-DR4-positive subjects. Whole peripheral blood mononuclear cells (PBMCs) of RA patients and control subjects were stimulated with citrullinated fibrinogen peptides, and T-cell production of proliferation and proinflammatory cytokines, such as interferon-gamma(IFN-gamma) and interleukin-17A (IL-17A), were measured. In addition, CD4 T cells from RA patients were stimulated with the citrullinated fibrinogen peptide, Fib-alpha R84Cit, identified as a DRB1*0401-restricted T cell epitope in HLA-DR4 transgenic mice, and the degree of T cell activation was examined similarly. No proliferative responses to the citrullinated fibrinogen peptides were observed in whole PBMCs or CD4 T cells from RA patients. Furthermore, no increased production of IFN-gamma or IL-17A was found in whole PBMCs or CD4 T cells stimulated with the citrullinated fibrinogen peptides, although these cells responded to recall antigen, a mixture of tetanus toxoid, purified protein derivative (PPD) from Mycobacterium tuberculosis, and Candida albicans. The results of this study indicate that anti-citrulline immunity in RA patients may be mediated by fibrinogen because there is no evidence of CD4 T cell-mediated immune responses to citrullinated fibrinogen peptides.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Arthritis, Rheumatoid
/
Candida albicans
/
Fibrinogen
/
Mice, Transgenic
/
Tetanus Toxoid
/
T-Lymphocytes
/
HLA-DR4 Antigen
/
Cytokines
/
Epitopes, T-Lymphocyte
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Immune Network
Year:
2013
Type:
Article
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