Biological Properties of Mesenchymal Stem Cells Derived from Induced Pluripotent Stem Cells / 中国实验血液学杂志

ABSTRACT

OBJECTIVE@#To obtain induced pluripatent stem cells (iPSC) from peripheral blood mononucleated cells and further induce differentiation into mesenchymal stem cells (MSC), and to compare the biological characteristics of iPSC-derived MSC and other-derived MSC.@*METHODS@#Peripheral blood mononucleated cells were obtained and transduced with reprogramming factors by sendai virus vector. Induced differentiation of MSC was performed in 1 strain of iPSC that completed all identification, and their cell morphology and immunophenotype were identified by immu-nohistochemistry and flow cytometry. Adipogenic and osteogenic media were used to induce the adipogenic and osteogenic differentiation. The expression of immune-related transcription factors was identified by PCR to systematically elucidate the biological characteristics of iPSC-induced MSC.@*RESULTS@#After transfection with sendai virus with reprogramming factor, the fate of peripheral blood mononucleated cells was reversed, initiating the expression of stem cell characteristics, the iPSC was successfully cloned, amplified, and purified, and finally the stable proliferation of iPSC was obtained. Mesenchymal stem cells derived from iPSC, had morphology consistent with other-derived MSC, and the immunophenotypes met the standard. iPSC-MSC possessed the ability of lipogenic and osteogenic differentiation. RT-PCR showed that iPSC-MSC was high expression to PDL1, and low expression to A20; besides, the expression level of STAT3 was equal to BM-MSC; and also as to the expression level of HIF1α and UC-MSC, which was lower than BM-MSC.@*CONCLUSION@#Peripheral blood mononucleated cells successfully initiated the expression of stem cell characteristics after the transduction of sendai virus vector with reprogramming factors, and obtained multi-competent iPSC. iPSC can successfully be induced to the differentiation of MSC, and the iPSC-MSC have standard cell morphology, immunophenotype and differentiation ability. High expression of PDL1 and low expression of A20 in iPSC-MSC suggest that iPSC-derived cells have different biological characteristics in cell proliferation and immune regulation.