Angelica Polysaccharide Resists Platelets Apoptosis Induced by LY294002 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1208-1214, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-775740
ABSTRACT
OBJECTIVE@#To investigate the anti-apoptotic effect of Angelica polysaccharide (APS) on cryopreservated platelets and its mechanism.@*METHODS@#The platelets were divided into 4 group control group(4 ℃ stored platelets),APS group (APS-treated platelets stored at 4 ℃), LY294002 group (LY294002-treated platelets stored at 4 ℃) and LY294002+APS group(LY294002+APS treated platelets stored at 4 ℃ ). The expression of platelet membrane glycoprotein CD41 and CD61, as well as the platelet apoptotic rate, Caspase 3 expression and mitochondrial membrane potential (MMP) were detected by flow cytometry; the anti-apoptotic mechanism of APS by PI3K /AKT signaling pathway was analyzed by Western blot assay.@*RESULTS@#The apoptosis rate of platelets in LY294002 group obviously increased, the activity of CD41 and CD61 expression gradually decreased along with the enhancement of LY294002 concentrations (r=-0.953); compared with control group, the apoptosis rate of platelets in LY294002 group was enhanced significantly(P<0.05),while the apoptosis rate of platelets in LY294002+APS group significantly was reduced(P<0.05) as compare with LY294002 group, which suggest that APS has an anti-apoptotic effect on the cryopreserved platelets. APS decreased the expression of Caspase-3 and inhibited the reduction of mitochondrial membrane potential induced by LY294002, moreover, APS could increase the activation of PI3K /AKT pathway in Plt.@*CONCLUSION@#APS has an anti-apoptotic effect on the cryopreserved platelets through activating the PI3K /AKT pathway, decreasing the expression of apoptosis protease Caspase-3 and inhibiting the reduction of MMP.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polysaccharides
/
Blood Platelets
/
Morpholines
/
Chromones
/
Apoptosis
/
Phosphatidylinositol 3-Kinases
/
Angelica
/
Proto-Oncogene Proteins c-akt
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2019
Type:
Article
Similar
MEDLINE
...
LILACS
LIS