Sequencing of a large off-ladder allele at Penta E locus / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 168-170, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-775786
ABSTRACT
OBJECTIVE@#To identify a rare large off ladder (OL) allele at the Penta E locus.@*METHODS@#Chelex-100 was used to extract DNAs from the blood samples. The PCR fragments were purified, extracted, and subjected to TA cloning and sequencing.@*RESULTS@#An OL allele was identified by the PowerPlex™ 21 at the Penta E locus, which was postulated as allele 26 based on assigned size. The OL allele was verified as a novel fragment containing 26 full AAAGA repeats.@*CONCLUSION@#OL alleles and microvariants should be verified by direct sequencing. Typing of OL alleles has significance for both daily work and forensic genetics.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymerase Chain Reaction
/
Rubiaceae
/
Alleles
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2019
Type:
Article
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