De novo construction of mammalian synthetic inhibitory transcription factor and promoter pairs / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1886-1894, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-776280
ABSTRACT
Transcriptional regulation is crucial for regulated gene expression. Due to the complexity, it has been difficult to engineer eukaryotic transcription factor (TF) and promoter pairs. The few availabilities of eukaryotic TF and promotor pairs limit their practical use for clinical or industrial applications. Here, we report a de novo construction of synthetic inhibitory transcription factor and promoter pairs for mammalian transcriptional regulation. The design of synthetic TF was based on the fusion of DNA binding domain and Kruppel associated box transcription regulating domain (KRAB). The synthetic promoter was constructed by inserting the corresponding TF response element after SV40 promoter. We constructed and tested five synthetic inhibitory transcription factor and promoter pairs in cultured mammalian cells. The inhibition capability and orthogonality were verified by flow cytometry. In summary, we demonstrate the feasibility of constructing mammalian inhibitory TF and promoter pairs, which could be standardized for advanced gene-circuit design and various applications in the mammalian synthetic biology.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
Transcription, Genetic
/
Gene Expression Regulation
/
Promoter Regions, Genetic
/
Gene Regulatory Networks
/
Mammals
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2018
Type:
Article
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