Identification of the core promoter of the pmel gene of Bashang long-tail chickens / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1750-1759, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-776293
ABSTRACT
To explore the activity of the pmel core promoter of Bashang long-tail chickens, we constructed dual-luciferase expression vectors and transiently transfected into DF1 cells with Lipofectamine 2000. We measured the luciferase activity with the dual-luciferase detection kit. The 1 268 bp fragment in 5-flanking region of the pmel gene in Bashang long-tail chickens was cloned. The region from -1 200 bp to +68 bp included 2 CpG islands and multiple transcription factor binding sites. We constructed 9 expression vectors with different promoter regions and a mutant vector of the core promoter region of the pmel gene of Bashang long-tail chickens. The core promoter region from -840 bp to +68 bp was identified in the pmel gene. The region from -590 to -525 bp negatively regulated the pmel gene during the transcription process. The -840--590 bp and -525--266 bp regions were positive regulatory regions. The polymorphic sites (-456, -435, -410, -374 and -341) had a significant effect on the promoter activity of the pmel gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chickens
/
Promoter Regions, Genetic
/
Cloning, Molecular
/
CpG Islands
/
Gp100 Melanoma Antigen
/
Genetics
/
Luciferases
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2018
Type:
Article
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