Identification of chemical constituents in Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 123-133, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-776413
ABSTRACT
This experiment was performed to analyze and identify the chemical constituents of Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn. The analysis was performed on an Agilent Zorbax SB-C₁₈ (4.6 mm×250 mm, 5 μm) column.The mobile phase consisted of 0.1% formic acid was used for gradient at a flow rate of 1.0 mL·min⁻¹. Electrospray ionization ion trap time-of-flight multistage mass spectrometry was applied for qualitative analysis under positive and negative ion modes. The results indicated that 54 compounds consisted of 18 lignans and 36 flavonoids from Xiaoyelian had been detected by their HRMS data, the information of literature and reference substance. Among them, 27 compounds were reported in Sinopodophylli Fructus for the first time. In conclusion, an HPLC-DAD-ESI-IT-TOF-MSn method was established to qualitative analysis of Xiaoyelian in this study, which will provide the evidence for evaluating the quality of Xiaoyelian herbs, clarifying the mechanism, and guiding the development of pharmacological active ingredients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Flavonoids
/
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Lignans
/
Spectrometry, Mass, Electrospray Ionization
/
Berberidaceae
/
Tandem Mass Spectrometry
/
Phytochemicals
/
Fruit
Type of study:
Diagnostic study
/
Qualitative research
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2018
Type:
Article
Similar
MEDLINE
...
LILACS
LIS