High-Throughput Automatic Training System for Spatial Working Memory in Free-Moving Mice / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 389-400, 2019.
Article
in English
| WPRIM
| ID: wpr-776473
ABSTRACT
Efficient behavioral assays are crucial for understanding the neural mechanisms of cognitive functions. Here, we designed a high-throughput automatic training system for spatial cognition (HASS) for free-moving mice. Mice were trained to return to the home arm and remain there during a delay period. Software was designed to enable automatic training in all its phases, including habituation, shaping, and learning. Using this system, we trained mice to successfully perform a spatially delayed nonmatch to sample task, which tested spatial cognition, working memory, and decision making. Performance depended on the delay duration, which is a hallmark of working memory tasks. The HASS enabled a human operator to train more than six mice simultaneously with minimal intervention, therefore greatly enhancing experimental efficiency and minimizing stress to the mice. Combined with the optogenetic method and neurophysiological techniques, the HASS will be useful in deciphering the neural circuitry underlying spatial cognition.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Behavior, Animal
/
Equipment Design
/
Automation, Laboratory
/
Spatial Memory
/
Habituation, Psychophysiologic
/
Memory, Short-Term
/
Mice, Inbred C57BL
Limits:
Animals
Language:
English
Journal:
Neuroscience Bulletin
Year:
2019
Type:
Article
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