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Effects of Notch signal on the expressions of HIF-α and autophagy- related genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation induced myocardial cell injury / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 165-168, 2019.
Article in Chinese | WPRIM | ID: wpr-776540
ABSTRACT
OBJECTIVE@#To investigate the effects of Notch signal on hypoxic induction factor (HIF-1α) and autophagy-associated genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation (OGD) induced myocardial cell injury.@*METHODS@#The OGD model was established using hypoxic culture box and hypoglycemic DMEM medium. The cells were divided into normal control group, OGD group, OGD + NC siRNA group, OGD + Notch1 siRNA group and OGD + HIF-1α siRNA group. Western blot was used to detect the interference effects of HIF-1α siRNA and Notch1 siRNA. The effects of Notch1 siRNA and HIF-1α siRNA on the activity of myocardial cells in OGD model were detected by the CCK-8 assay. The effects of Notch1 siRNA and HIF-1α siRNA on autophage-associated genes Beclin1, LC3I and LC3II expression were detected by Western blot.@*RESULTS@#The results of Western blot showed that HIF-1α siRNA could effectively knock down the expression of HIF-1α in myocardial cells in OGD model, and Notch1 siRNA could effectively knock down the expression of Notch1 and HIF-1α in myocardial cells in OGD model. The result of CCK-8 assay showed that Notch1 siRNA and HIF-1α siRNA reduced the activity of myocardial cells in OGD model, and there was no statistical difference between the two groups. Western blot results showed that Notch1 siRNA and HIF-1α siRNA could reduce the expressions of the autophagy-associated genes Beclin1, LC3I and LC3II, and reduce the ratio of LC3II to LC3I at mRNA level.@*CONCLUSION@#Notch1 plays a role in myocardial protection by regulating the expression of HIF-1α to regulate the autophagy in OGD model cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Pathology / Autophagy / Signal Transduction / Cell Hypoxia / Cells, Cultured / Cell Biology / Myocytes, Cardiac / Hypoxia-Inducible Factor 1, alpha Subunit / Receptors, Notch Type of study: Prognostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Applied Physiology Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Pathology / Autophagy / Signal Transduction / Cell Hypoxia / Cells, Cultured / Cell Biology / Myocytes, Cardiac / Hypoxia-Inducible Factor 1, alpha Subunit / Receptors, Notch Type of study: Prognostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Applied Physiology Year: 2019 Type: Article