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Anti-fibrotic Effects and Mechanism of Shengmai Injection () on Human Hepatic Stellate Cells LX-2 / 中国结合医学杂志
Chinese journal of integrative medicine ; (12): 197-202, 2019.
Article in English | WPRIM | ID: wpr-776615
ABSTRACT
OBJECTIVE@#To investigate the effects of Shengmai Injection (, SMI) on the proliferation, apoptosis and N-myc downstream-regulated gene 2 (NDRG2, a tumour suppressor gene) expression in varying densities of human hepatic stellate cells LX-2.@*METHODS@#LX-2 cells were cultured in vitro. Then, cells were plated in 96-well plates at an approximate density of 2.5×10 cells/mL and cultured for 48, 72, 96 or 120 h followed by the application of different concentrations of SMI (0.6, 1.2, 2.4, 4.8 or 6 μL/mL). Cell proliferation was measured after an additional 24 or 48 h using the 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of SMI on different cell growth states (cultured for 48, 72, 96, or 120 h) were observed by light microscopy at 24 h after treatment. When the cells reached 80% conflfluence, apoptosis was detected by flflow cytometry after 24 h. Lastly, LX-2 cells were treated with different concentrations of SMI and extracted with protein lysis buffer. The levels of NDRG2 were measured by Western blot.@*RESULTS@#When the LX-2 cells grew for 48, 72, 96 and 120 h, 4.8 and 6 μL/mL of SMI significantly inhibited cell proliferation at 24 and 48 h after treatment (P<0.05). And 2.4 μL/mL of SMI also inhibited cell proliferation at 24 h after treatment when cell growth for 48 h (P<0.05) and at 48 h after treatment when cell growth for 72, 96 and 120 h (P<0.05). The NDRG2 expression level in the LX-2 cell was significantly increased when treated with SMI at concentrations of 1.2, 2.4, 4.8 or 6 μL/mL (P<0.05).@*CONCLUSION@#The inhibitory effects of SMI on the proliferation of LX-2 cells were related to not only concentration dependent but also cell density. In addition, SMI (2.4, 4.8 and 6 μL/mL) could accelerate apoptosis in LX-2 cells, and the mechanism might be associated with NDRG2 over-expression.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Drugs, Chinese Herbal / Cells, Cultured / Apoptosis / Tumor Suppressor Proteins / Cell Proliferation / Drug Therapy / Hepatic Stellate Cells / Genetics Limits: Humans Language: English Journal: Chinese journal of integrative medicine Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Drugs, Chinese Herbal / Cells, Cultured / Apoptosis / Tumor Suppressor Proteins / Cell Proliferation / Drug Therapy / Hepatic Stellate Cells / Genetics Limits: Humans Language: English Journal: Chinese journal of integrative medicine Year: 2019 Type: Article