Clinical value of droplet digital PCR in rapid diagnosis of invasive fungal infection in neonates / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 45-51, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-776655
ABSTRACT
OBJECTIVE@#To evaluate the clinical value of droplet digital PCR (ddPCR) in rapid and accurate diagnosis of invasive fungal infection (IFI) in neonates.@*METHODS@#The highly conserved sequence of fungi 18S RNA was selected as the target sequence, and primers were designed to establish a ddPCR fungal detection system. Blood samples were collected from 83 neonates with high-risk factors for IFI and/or related clinical symptoms in the neonatal intensive care unit (NICU) of a hospital in Shenzhen, China. Blood culture and ddPCR were used for fungal detection.@*RESULTS@#The ddPCR fungal detection system had a specificity of 100% and a sensitivity of 3.2 copies/μL, and had a good reproducibility. Among the 22 blood samples from neonates with a confirmed or clinical diagnosis of IFI, 19 were detected positive by ddPCR. Among the 61 blood samples from neonates who were suspected of IFI or had no IFI, 2 were detected positive by ddPCR.@*CONCLUSIONS@#The ddPCR technique can be used for the detection of neonatal IFI and is a promising tool for the screening and even diagnosis of neonatal IFI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
China
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
Invasive Fungal Infections
Type of study:
Diagnostic study
/
Risk factors
Limits:
Humans
/
Infant, Newborn
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2019
Type:
Article
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