Sequence analysis and 3D molecular structure simulation of a novel HLA allele B*51:159 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 1133-1135, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-776728
ABSTRACT
OBJECTIVE@#To identify a novel human leukocyte antigen (HLA) B allele in a Chinese Han individual and construct its three-dimensional structure.@*METHODS@#The initial HLA genotyping was performed by PCR-sequence-based typing (PCR-SBT). The ambiguous allele was confirmed with single-strand DNA sequencing. The DNA sequence was analyzed to identify the difference between the novel allele and its closest matching allele. Finally, the three-dimensional molecular structure of the novel allele was constructed using a Swiss-Model.@*RESULTS@#One allele of the subject at the HLA-B locus was B*440301, whilst the other was a novel allele which differed from the closest matching allele B*51010101 by nucleotide (nt) 329 A to C in exon 2, resulting in an amino acid change at codon 86 (p.Asn86Thr).@*CONCLUSION@#A novel HLA-B allele has been identified and officially named as HLA-B*51159 by the WHO Nomenclature Committee for Factors of the HLA System. The three-dimensional structure of B*51159 was simulated.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Base Sequence
/
HLA-B Antigens
/
Chemistry
/
Sequence Analysis, DNA
/
Asian People
/
Alleles
/
Genetics
/
Molecular Conformation
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2019
Type:
Article
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