Identification of stable reference gene by qRT-PCR in Senecio scandens / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 465-471, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-777478
ABSTRACT
As a traditional Chinese medicine, Senecio scandens is rich in important compounds such as flavonoid and sesquiterpenoid. Based on the transcriptome data of S. scandens, 15 candidate reference genes were selected including ABCT, ACT1, ACT2, ACT3, ACBP, ARF, ATPS, EF-H, EF-1α, ETIF, GAPDH, GTPB, MPS, UCE and 60S. Firstly, 9 candidate genes with relatively stable expressions such as ACT1, ACBP, ARF, ATPS, EF-1α, GAPDH, MPS, UCE and 60S were screened from different tissues of S. scandens by RT-PCR. Then, qRT-PCR was used to quantitatively analyze gene expression of these nine candidates in S. scandens with or without stress treatments. Further analysis of these gene expression data by geNorm and NormFinder showed that ACT1 exhibited the stablest expression in all samples and could serve as a reference gene for future study of S. scandens, and provide an endogenous control for gene expression analysis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Reference Standards
/
Senecio
/
Genes, Plant
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gene Expression Profiling
/
Transcriptome
/
Genetics
/
Medicine, Chinese Traditional
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2019
Type:
Article
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