Separation and identification of glycoprotein in human serum of Fragile X syndrome
Journal of Medical Research
;
: 22-28, 2008.
Article
in Vietnamese
| WPRIM
| ID: wpr-778
ABSTRACT
Background:
Fragile X Syndrome (FXS) is the most common cause of inherited mental retardation. The absence of Fragile X Mental Retardation (FMRP) in Fragile X syndrome changes other proteins.Objective:
To detect changes of glycoprotein in human serum of Fragile X syndrome. Subject andmethods:
Affinity chromatography with lectin concanavalin A (ConA) used to receive glycoprotein. The collected glycoprotein was then separated using 2-D electrophoresis. The protein spots were further excised, trypsin digested, and analyzed by nano LC couple with ESI-MS/MS and identified by MASCOT v1.8 software. Results andconclusion:
5 glycoproteins showed the different expression levels in the serum of Fragile X syndrome. Haptoglobin, Ig-J were increased and ceruloplasmin, transferring, Ig kappa were decreased. Using affinity chromatography with lectin concanavalin A (ConA), glycoprotein was received and divided on 2 ways electrokinetic chromatography. The mixture protein was identified with a reliability of 99.5% by 2 ways liquid chromatography combined with continuous spectrum mass.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proteomics
/
Fragile X Syndrome
Type of study:
Diagnostic study
Language:
Vietnamese
Journal:
Journal of Medical Research
Year:
2008
Type:
Article
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