Expression and significance of Bax, COX2, and ID1 in gallbladder adenocarcinoma / 临床肝胆病杂志

ABSTRACT

ObjectiveTo investigate the expression and significance of Bax, cyclooxygenase-2 (COX2), and inhibitor of differentiation 1 (ID1) in gallbladder adenocarcinoma tissues. MethodsA total of 70 patients confirmed with gallbladder adenocarcinoma by pathological examination in the Department of Pathology, The First Hospital of Lanzhou University, from 2000 to 2013 were collected. Besides, 20 cases of high-grade intraepithelial neoplasia, 30 cases of low-grade intraepithelial neoplasia, and 20 cases of cholecystitis were selected as controls. The protein expression of Bax, COX2, and ID1 in the four groups was determined using the immunohistochemical SP method. Meanwhile, chi-square test was conducted on the data using fourfold table exact test, the correlations between Bax, COX2, and ID1 were determined by Spearman correlation analysis, and survival data were subjected to Kapan-Meier survival analysis. ResultsThe high-grade intraepithelial neoplasia group, low-grade intraepithelial neoplasia group, and cholecystitis group showed significantly different expression of Bax compared with the gallbladder adenocarcinoma group (all P＜0.05); the low-grade intraepithelial neoplasia group and cholecystitis group showed significantly different expression of COX2 and ID1 compared with the gallbladder adenocarcinoma group (all P＜0.05); the high-grade intraepithelial neoplasia group showed significantly different expression of COX2 and ID1 compared with low-grade intraepithelial neoplasia group and cholecystitis group (all P＜0.05). Survival analysis showed that patients with positive Bax had a significantly higher survival rate than those with positive COX2 and ID1 (both P＜0.05). COX2 was positively correlated with ID1 (r=0.329, P＜0.05). ConclusionBax, COX2, and ID1 may play a role in the development of gallbladder adenocarcinoma, and joint detection of Bax, COX2, and ID1 is of great significance for the prognosis of gallbladder adenocarcinoma.