Effect of Pien Tze Huang on bile excretion and its analgesic effect in experimental animals / 临床肝胆病杂志

ABSTRACT

ObjectiveTo investigate the effect of Pien Tze Huang on bile excretion and its analgesic effect in experimental animals. MethodsA total of 30 guinea pigs were randomly divided into Pien Tze Huang group, ursodeoxycholic acid group, and control group. According to body mass, the three groups were treated with Pien Tze Huang (140 mg/kg), ursodeoxycholic acid (22.5 mg/kg), and normal saline, respectively, by gavage for 4 consecutive days. Then bile drainage was performed to measure the volume of bile secretion and the change in bile composition. Related liver function parameters were also measured. A total of 30 mice were randomly divided into Pien Tze Huang group (360 mg/kg), ursodeoxycholic acid group (120 mg/kg), and control group. At one hour after the last administration, the mice were given intraperitoneal injection of 0.6% glacial acetic acid (0.15 ml/10 g), and the writhing test was performed to observe the analgesic effect of Pien Tze Huang. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the control group, the Pien Tze Huang group and the ursodeoxycholic acid group had a significant increase in the volume of bile secretion (P=0.039 and 0.009). There were no significant differences between the three groups in cholesterol, bilirubin, total bile acid, and phospholipid in bile (all P＞0.05) and liver function parameters (alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, total bilirubin, direct bilirubin, indirect bilirubin, and total bile acid) (all P＞0.05). Compared with the control group, the Pien Tze Huang group had a significant reduction in the number of writhing times (P＜0.001), suggesting that Pien Tze Huang had a marked analgesic effect. ConclusionAnimal experiments show that Pien Tze Huang has marked choleretic and analgesic effects, which provides theoretical and data support for the clinical application of Pien Tze Huang.