Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice / 临床肝胆病杂志

ABSTRACT

ObjectiveTo investigate the expression of D-bifunctional protein (DBP) in hepatocellular carcinoma (HCC) tissue in rats and the growth of DBP-induced HCC in nude mice. MethodsA total of 22 male Sprague-Dawley rats were randomly divided into normal control group with 8 rats and model group with 14 rats treated with intraperitoneally injected diethylnitrosamine to induce HCC, and Western blotting, immunohistochemistry, and RT-PCR were used to measure the expression of DBP. A total of 14 specific pathogen-free male BALB/c-nu mice were randomly divided into two groups. HepG2 cells were transfected with empty plasmid or DBP overexpression plasmid and were then injected subcutaneously into nude mice. There were 8 mice in the empty plasmid control group and 6 mice in the DBP high-expression plasmid group, and tumor size was measured for both groups. The t-test was used for comparison of continuous data between groups. ResultsThe rats with HCC had significantly higher protein and mRNA expression of DBP in liver tissue than normal rats (protein 1.10±0.35 vs 0.67±0.12, t=-7.48, P＜0.05; mRNA 3.70±0.85 vs 1.17±0.72, t=-20.46, P＜0.05). The DBP high-expression plasmid group had a significantly higher tumor volume than the empty plasmid group ［(7590.50±1867.97)mm3 vs (1663.78±420.24)mm3, t=-39.78, P＜0.01］. ConclusionHighly expressed DBP can promote the progression of HCC in rats and thus provides a new target for the treatment of HCC and the research and development of inhibitory drugs.