Rapid analysis of glycerophospholipids in RAW264.7 macrophage with UHPLC-QTOF/MS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1451-1457, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-779570
ABSTRACT
An ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometric method was developed for rapid analysis of glycerophospholipids in RAW264.7 macrophage. The modified Bligh-Dyer was applied to extract glycerophospholipids from RAW264.7 macrophage. The target compounds, detected by mass spectrometry in ESI+ and ESI- mode, were separated by gradient elution with mobile phase (A) water (containing 10mmol·L-1 ammonium acetate and 0.25% acetic acid) and (B) acetonitrile/isopropanol (11) (containing 10mmol·L-1 ammonium acetate and 0.25% acetic acid). A total of 82 glycerophospholipids including 57 phosphatidylcholines (PCs), 21 phosphatidylethanolamines (PEs), three phosphatidylglycerols (PGs) and one phosphatidylinositol (PI) were deduced. The UHPLC-QTOF/MS method is rapid, simple and credible for targeting analysis of glycerophospholipids of RAW264.7 macrophage.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2016
Type:
Article
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