Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/ Reperfusion Injury
Malaysian Journal of Medical Sciences
;
: 39-46, 2019.
Article
in English
| WPRIM
| ID: wpr-780765
ABSTRACT
@#Backgrounds Renal ischemia/reperfusion (RIR) is a major cause of kidney dysfunction in clinic. The main objective of this study was to investigate the effect of pre-conditioning ischemia (IPC) and zinc (Zn) supplementation on renal RIR injury. Methods:
A total of 63 unilateral nephrectomised male and female Wistar rats were divided into five groups. Group 1 (ShOPR) Rats as sham-operated group were subjected to surgical procedure without RIR. Group 2 (Isch) Rats underwent RIR (left kidney ischemia for 30 min followed by 48 h reperfusion). Group 3 (Zn+Isch) Rats were treated as group 2 but they received Zn sulphate (30 mg/kg) 1 h before induction of RIR. Group 4 (IPC+Isch) Rats were treated as group 2 but they underwent 1 min of ischemia followed by 3 min reperfusion as IPC, which was repeated for three times before induction of RIR. Group 5 (Zn+IPC+Isch) Rats were subjected to receive both Zn sulphate and IPC before induction of RIR. Urine samples were collected in the last 6 h of reperfusion, and finally biochemical and histological measurements were performed.Results:
The serum level of creatinine (Cr), normalised kidney weight (KW) and kidney tissue damage score (KTDS) increased by RIR alone significantly (P < 0.05). These parameters were attenuated statistically by Zn supplementation (P < 0.05). However, IPC alone or cotreatment of Zn and IPC did not improve the biochemical and histological markers altered by RIR injury.Conclusion:
Zn supplementation had a protective role against RIR while such protective effect was not observed by IPC alone or by co-treatment of Zn and IPC.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Malaysian Journal of Medical Sciences
Year:
2019
Type:
Article
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