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Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish / 中国结合医学杂志
Chinese journal of integrative medicine ; (12): 130-137, 2020.
Article in English | WPRIM | ID: wpr-781333
ABSTRACT
OBJECTIVE@#To determine the effects of Hydrangeae Dulcis Folium (EHDF) on physical stress, changes in the whole-body cortisol level and behaviour in zebrafish (Danio rerio).@*METHODS@#One hundred and seventy-four fish were randomly divided into 4 [adrenocorticotropin hormone (ACTH) challenge test 4 fish per group] or 6 groups (behavioural test 10-12 fish per group, whole-body cortisol 4 fish per group). Net handling stress (NHS) was used to induce physical stress. Fish were treated with vehicle or EHDF (5-20 mg/L) for 6 min before they were exposed to stress. And then, fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests, including novel tank test and open field test, and were evaluated to observe anxiety-like behaviours and locomotion. In addition, to elucidate the mode of action of the anti-stress effects of EHDF, ACTH (0.2 IU/g, i.p.) challenge test was performed.@*RESULTS@#The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5-20 mg/L (P <0.05). Moreover, compared with the unstressed group, which was not treated with NHS, the whole-body cortisol level was significantly increased by treatment with NHS (P <0.05). Compared with the NHS-treated stressed control group, pre-treatment with EHDF at concentrations of 5-20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level (<0.05). In addition, ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion (P <0.05).@*CONCLUSION@#EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.

Full text: Available Index: WPRIM (Western Pacific) Language: English Journal: Chinese journal of integrative medicine Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: English Journal: Chinese journal of integrative medicine Year: 2020 Type: Article