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Effect of the focal adhesion kinase inhibitor TAE226 on the epithelial-mesenchymal transition in human oral squamous cell carcinoma cell line / 华西口腔医学杂志
West China Journal of Stomatology ; (6): 17-22, 2020.
Article in Chinese | WPRIM | ID: wpr-781351
ABSTRACT
OBJECTIVE@#To study the effect of the focal adhesion kinase inhibitor TAE226 on epithelial-mesenchymal transition (EMT) in human oral squamous cell carcinoma (OSCC) cell line.@*METHODS@#HSC-3 and HSC-4 cells were cultured with TAE226 under different concentrations (0, 1, 5, and 10 μmol·L⁻¹) for 24, 48, and 72 h. Real-time quantitative polymerase chain reaction was performed to detect the mRNA expressions of E-cadherin and Vimentin. The protein expressions of E-cadherin and Vimentin were determined by Western blot assay after 48 h of TAE226 treatment.@*RESULTS@#Real-time quantitative polymerase chain reaction showed that increasing the TAE226 dose and reaction time resulted in increased and decreased E-cadherin and Vimentin mRNA expressions, respectively (P<0.05). Western blot assays showed that increasing the TAE226 dose resulted in increased and decreased E-cadherin and Vimentin protein expressions, respectively (P<0.05).@*CONCLUSIONS@#TAE226, which is expected to be an effective drug for OSCC treatment, can effectively inhibit the EMT of the OSCC cell line.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Vimentin / Mouth Neoplasms / Carcinoma, Squamous Cell / Cadherins / Morpholines / Cell Line, Tumor / Focal Adhesion Protein-Tyrosine Kinases / Epithelial-Mesenchymal Transition Limits: Humans Language: Chinese Journal: West China Journal of Stomatology Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Vimentin / Mouth Neoplasms / Carcinoma, Squamous Cell / Cadherins / Morpholines / Cell Line, Tumor / Focal Adhesion Protein-Tyrosine Kinases / Epithelial-Mesenchymal Transition Limits: Humans Language: Chinese Journal: West China Journal of Stomatology Year: 2020 Type: Article