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Janus kinase 3 facilitates the migration of breast cancer cells by store-operated calcium channel / 生理学报
Acta Physiologica Sinica ; (6): 874-882, 2019.
Article in Chinese | WPRIM | ID: wpr-781387
ABSTRACT
The present study was aimed to investigate the effect of Janus kinase 3 (JAK3) on the migration of breast cancer cells and the underlying mechanism. The expression of JAK3 in breast cancer MCF-7 cells was silenced by siRNA (siJAK3). The migration ability of MCF-7 cells was detected by scratch test. The activity of store-operated calcium channel (SOCC) was detected by fluorescence calcium imaging. The expression levels of Orai1 and STIM1, key molecules in the process of store-operated calcium entry (SOCE) were detected by Western blot and RT-PCR. The results showed that 2-APB, an inhibitor of SOCC, could inhibit the migration ability of MCF-7 cells. siJAK3 transfection significantly inhibited the migration ability of MCF-7 cells, decreased the activity of SOCC, and down-regulated mRNA and protein expression levels of Orai1 and Stim1. Over-expression of Orai1 or STIM1 in JAK3-silenced cells restored their migration ability. These results suggest that JAK3 facilitates the migration of breast cancer cells by SOCC.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Breast Neoplasms / Calcium Channels / Gene Expression Regulation, Neoplastic / Cell Movement / Calcium / Janus Kinase 3 / MCF-7 Cells / ORAI1 Protein / Genetics Limits: Humans Language: Chinese Journal: Acta Physiologica Sinica Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Breast Neoplasms / Calcium Channels / Gene Expression Regulation, Neoplastic / Cell Movement / Calcium / Janus Kinase 3 / MCF-7 Cells / ORAI1 Protein / Genetics Limits: Humans Language: Chinese Journal: Acta Physiologica Sinica Year: 2019 Type: Article