Practical stability of whole-genome bisulfite sequencing using plasma cell-free DNA / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2284-2294, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-781638
ABSTRACT
With the development of liquid biopsy technology, plasma cell-free DNA (cfDNA) becomes one of the research hotspots. Whole-genome bisulfite sequencing of plasma cell-free DNA has shown great potential medical applications such as cancer detection. However, the practical stability evaluation is still lacking. In this study, plasma cell-free DNA samples from two volunteers at different time were collected and prepared for sequencing in multiple laboratories. The library preparation strategies include pre-bisulfite, post-bisulfite and regular whole-genome sequencing. We established a set of quality control references for plasma cell-free DNA sequencing data and evaluated practical stability of blood collection, DNA extraction, and library preparation and sequencing depth. This work provided a technical practice guide for the application of plasma cfDNA methylation sequencing for clinical applications.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sulfites
/
Sequence Analysis, DNA
/
DNA Methylation
/
High-Throughput Nucleotide Sequencing
/
Cell-Free Nucleic Acids
/
Whole Genome Sequencing
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2019
Type:
Article
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