HER2-Positive Breast Carcinomas with Co-amplification or Gain of Chromosome 17 Centromere Locus: Report of Three Cases and an Impact on HER2 Testing
Korean Journal of Pathology
;
: 665-669, 2011.
Article
in English
| WPRIM
| ID: wpr-78174
ABSTRACT
Recently we experienced three cases of human epidermal growth factor receptor 2 (HER2)-amplified invasive breast carcinomas associated with co-amplification or gain of chromosome 17 centromere (CEP17) in silver-enhanced in situ hybridization (SISH) analysis. These cases revealed 2+ or 3+ staining for HER2 immunohistochemistry and >6 HER2 copies per cell on SISH analyses. However, the calculated HER2/CEP17 ratios were low (6 per cell vs HER2/CEP17 ratio>2.2). We recommend reporting raw SISH or fluorescence in situ hybridization data, including number of cells counted, average numbers of HER2 and CEP17 signals, and the calculated HER2/CEP17 ratio to prevent underreporting of HER2 amplification.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chromosomes, Human, Pair 17
/
Breast
/
Breast Neoplasms
/
Immunohistochemistry
/
Centromere
/
In Situ Hybridization
/
Receptor, ErbB-2
/
Coat Protein Complex I
/
ErbB Receptors
/
Fluorescence
Limits:
Humans
Language:
English
Journal:
Korean Journal of Pathology
Year:
2011
Type:
Article
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