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Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip
Article in English | WPRIM | ID: wpr-782521
Responsible library: WPRO
ABSTRACT
detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Bees / RNA / RNA, Viral / Polymerase Chain Reaction / Sensitivity and Specificity / DNA, Complementary / Diagnosis / Beekeeping / Methods Language: English Journal: Journal of Veterinary Science Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bees / RNA / RNA, Viral / Polymerase Chain Reaction / Sensitivity and Specificity / DNA, Complementary / Diagnosis / Beekeeping / Methods Language: English Journal: Journal of Veterinary Science Year: 2020 Type: Article