Effects of Peroxisome Proliferator-Activated Receptor-gamma on the Production of Tumor Necrosis Factor-alpha in Stimulated Human Monocoytes / 감염과화학요법
Infection and Chemotherapy
;
: 291-295, 2010.
Article
in Korean
| WPRIM
| ID: wpr-78360
ABSTRACT
BACKGROUND:
We evaluated the effects of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on the production of tumor necrosis factor-alpha (TNF-alpha) and expression of nuclear factor-kappaB (NF-kappaB) in stimulated THP-1 cells, a human monocyte cell line. MATERIALS ANDMETHODS:
We evaluated the cytotoxic effect of 15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), one of natural PPAR-gamma ligands, using commercial cell proliferation assay. Cells were pretreated with 15d-PGJ(2) and then stimulated with lipopolysaccharide (LPS) or lipoteichoic acid (LTA). The amount of TNF-alpha was measured by using commercial ELISA method. NF-kappaB activation was evaluated by Western blot analysis.RESULTS:
15d-PGJ(2) showed dose-dependent cytotoxic effect on the tested cells after 4 hr of treatment. Stimulation of cells by LPS or LTA induced TNF-alpha production. TNF-alpha production was markedly decreased in the cells pretreated with 15d-PGJ(2) compared to cells treated only with LPS or LTA in a dose-dependent manner. Pretreatment of 15d-PGJ(2) reduced LPS or LTA induced NF-kappaB expression in the nuclear extracts of THP-1 cells.CONCLUSION:
15d-PGJ(2) pretreatment decreased TNF-alpha production from the THP-1 cells stimulated by LPS or LTA, and this assumed to be associated with inhibition of NF-kappaB activation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Teichoic Acids
/
Enzyme-Linked Immunosorbent Assay
/
Monocytes
/
Cell Line
/
Lipopolysaccharides
/
Blotting, Western
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Peroxisomes
/
Cell Proliferation
Limits:
Humans
Language:
Korean
Journal:
Infection and Chemotherapy
Year:
2010
Type:
Article
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