In Vitro Study On The Cytotoxicity Of The Differently Sterilized Demineralized Bone Powder

ABSTRACT

freezing. freeze-drying, and demineralization of the allogeneic bone must be under the germ-free stable condition without bacterial and/or viral contamination. Even though the bone is procured under the germ free condition, we must have confidence on disinfection of all the solutions that come in contact with tissue during the whole procedure. Lots of antibacterial agents have been introduced for chemical sterilization. Recently ethylene oxide gas sterilization or radiation sterilization is frequently selected as a secondary sterilization procedure. The biological and biochemical response of the graft material differs with the type and concentration of the sterilization agents, and various toxic reactions have been reported due to the graft material itself and the substance released by the chemicals. The authors conducted the Millipore filter test to observe the toxic effect on L929 fibroblasts according to the effect on activity of succinate dehydrogenase, during the toxic effect on L929 fibroblasts according to the effect on activity of succinate dehydrogenase. during the secondary sterilization of the demineralized allogeneic bone powder with irradiation or ethylene oxide gas. The results were as follows ; 1. Around the copper disk, positive control group, 10mm diameter discoloration was observed. 2. As same as the negative control group, the silicine disk showed no discoloration. 3. The demineralized allogeneic bone which was sterilized with ethylene oxide gas or irradiation showed no cytotoxicity. 4. From this results, it is suggested that treatment with ethylene oxide gas or irradiation should be effective to sterilize the demineralized allogeneic bone.