Proliferation and Functional Activity of Human Adipose Tissue-Derived CD146 Positive Endothelial Cells According to Culture Mediums
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
;
: 504-510, 2010.
Article
in Korean
| WPRIM
| ID: wpr-785031
ABSTRACT
PURPOSE: This study was to examine the proliferation and function of the adipose tissue-derived endothelial cells according to different culture medium conditions.MATERIALS AND METHODS:
Adipose tissue-derived CD146 positive endothelial cells were cultured in according to different culture mediums (DMEM culture medium with or without osteogenic inductive agents and EBM-2 culture medium with or without osteogenic inductive agents). The proliferation and function of the adipose tissue-derived endothelial cells was examined in different culture medium conditions.RESULTS:
Adipose tissue-derived endothelial cells formed tube-like structures on Matrigel in EBM-2 culture medium with or without osteogenic inductive agents. However, the cells did not form tube-like structures on Matrigel in DMEM medium with or without osteogenic inductive agents. After 24 hours of culture, among the culture medium using EBM-2, the proliferation of the cells were promoted in EBM-2 medium without osteogenic inductive agents than in EBM-2 medium with osteogenic inductive agents. However, 72 hours of culture, the proliferation of the cells were promoted in EBM-2 medium with osteogenic inductive agents than in EBM-2 medium without osteogenic inductive agents.CONCLUSION:
These results suggest that the proliferation and function of the adipose tissue-derived CD146 positive endothelial cells could be maintained in EBM-2 with osteogenic inductive agents.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proteoglycans
/
Collagen
/
Laminin
/
Durapatite
/
Endothelial Cells
/
Drug Combinations
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
Year:
2010
Type:
Article
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