Inhibition of Lymphatic Endothelial Growth Factor Receptor in a Murine Model of Oral Squamous Cell Carcinoma
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
;
: 1-9, 2011.
Article
in Korean
| WPRIM
| ID: wpr-785048
ABSTRACT
PURPOSE: Tumor associated angiogenesis and/or lymphangiogenesis are known to be linked by VEGFR signaling pathways. These processes are regulated by several growth factors including VEGFR-2, VEGFR-3. E7080 is an orally active inhibitor of multiple tyrosine kinases including VEGFR-2, 3. Therefore, it was proposed that E7080 may inhibit angiogenesis and lymphangiogenesis. The aim of this study was to determine the effect of E7080 in a nude mouse model of OSCC.METHODS:
KB cells were xenografted into the submucosal tissue of the mouth floor of athymic mice. Seven days after the xenograft, the mice were randomized into 2 groups. E7080 were administered orally to the experimental group once per day. The mice were sacrificed 3 weeks after the treatment. The tumors were examined histopathologically. Immunohistochemical assays with anti- VEGF-C, VEGFR-2, VEGFR-3, phosphorylated VEGFR-2/3 (pVEGFR-2/3), and D2-40 antibodies were then performed.RESULTS:
The transplantation of human OSCC tumor cells into the mouth floor resulted in the formation of orthotopic tumors. The experimental (E7080 treatment) group showed a slowly increased tumor volume. Moreover, immunohistochemical staining demonstrated higher levels of VEGF-C, VEGFR-2, VEGFR-3, pVEGFR-2/3 and D2-40 expression in the control group than in the experimental group.CONCLUSION:
These results suggest that E7080 may provide therapeutic benefits in OSCC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Tyrosine
/
Transplantation, Heterologous
/
KB Cells
/
Carcinoma, Squamous Cell
/
Endothelial Growth Factors
/
Transplants
/
Receptors, Vascular Endothelial Growth Factor
/
Vascular Endothelial Growth Factor Receptor-2
/
Vascular Endothelial Growth Factor Receptor-3
Type of study:
Controlled clinical trial
/
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
Year:
2011
Type:
Article
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