In vivo Osteogenesis of Cultured Human Periosteal-derived Cells and Polydioxanone/Pluronic F127 Scaffold
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
;
: 384-390, 2012.
Article
in Korean
| WPRIM
| ID: wpr-785191
ABSTRACT
PURPOSE: The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold.METHODS:
Two one-year-old miniature pigs were used in this study. 2x106 periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco's modified Eagle's medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed.RESULTS:
New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery.CONCLUSION:
These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Osteogenesis
/
Polyethylenes
/
Polypropylenes
/
Seeds
/
Azaperone
/
Swine
/
Durapatite
/
Anesthesia, General
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
Year:
2012
Type:
Article
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