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Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact
Clinical and Experimental Emergency Medicine ; (4): 125-137, 2019.
Article in English | WPRIM | ID: wpr-785606
ABSTRACT

OBJECTIVE:

Sequelae of behavioral impairments associated with human traumatic brain injury (TBI) include neurobehavioral problems. We compared exploratory, cognitive, and depressive-like behaviors in pediatric and adult male mice exposed to controlled cortical impact (CCI).

METHODS:

Pediatric (21 to 25 days old) and adult (8 to 12 weeks old) male C57Bl/6 mice underwent CCI at a 2-mm depth of deflection. Hematoxylin and eosin staining was performed 3 to 7 days after recovery from CCI, and injury volume was analyzed using ImageJ. Neurobehavioral characterization after CCI was performed using the Barnes maze test (BMT), passive avoidance test, open-field test, light/dark test, tail suspension test, and rotarod test. Acutely and subacutely (3 and 7 days after CCI, respectively), CCI mice showed graded injury compared to sham mice for all analyzed deflection depths.

RESULTS:

Time-dependent differences in injury volume were noted between 3 and 7 days following 2-mm TBI in adult mice. In the BMT, 2-mm TBI adults showed spatial memory deficits compared to sham adults (P < 0.05). However, no difference in spatial learning and memory was found between sham and 2-mm CCI groups among pediatric mice. The open-field test, light/dark test, and tail suspension test did not reveal differences in anxiety-like behaviors in both age groups.

CONCLUSION:

Our findings revealed a graded injury response in both age groups. The BMT was an efficient cognitive test for assessing spatial/non-spatial learning following CCI in adult mice; however, spatial learning impairments in pediatric mice could not be assessed.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Brain Injuries / Eosine Yellowish-(YS) / Hindlimb Suspension / Rotarod Performance Test / Spatial Learning / Spatial Memory / Hematoxylin / Learning / Memory Limits: Animals / Humans / Male Language: English Journal: Clinical and Experimental Emergency Medicine Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Brain Injuries / Eosine Yellowish-(YS) / Hindlimb Suspension / Rotarod Performance Test / Spatial Learning / Spatial Memory / Hematoxylin / Learning / Memory Limits: Animals / Humans / Male Language: English Journal: Clinical and Experimental Emergency Medicine Year: 2019 Type: Article