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Direct Reprogramming to Human Induced Neuronal Progenitors from Fibroblasts of Familial and Sporadic Parkinson’s Disease Patients
International Journal of Stem Cells ; : 474-483, 2019.
Article in English | WPRIM | ID: wpr-785826
ABSTRACT
In Parkinson’s disease (PD) research, human neuroblastoma and immortalized neural cell lines have been widely used as in vitro models. The advancement in the field of reprogramming technology has provided tools for generating patient-specific induced pluripotent stem cells (hiPSCs) as well as human induced neuronal progenitor cells (hiNPCs). These cells have revolutionized the field of disease modeling, especially in neural diseases. Although the direct reprogramming to hiNPCs has several advantages over differentiation after hiPSC reprogramming, such as the time required and the simple procedure, relatively few studies have utilized hiNPCs. Here, we optimized the protocol for hiNPC reprogramming using pluripotency factors and Sendai virus. In addition, we generated hiNPCs of two healthy donors, a sporadic PD patient, and a familial patient with the LRRK2 G2019S mutation (L2GS). The four hiNPC cell lines are highly proliferative, expressed NPC markers, maintained the normal karyotype, and have the differentiation potential of dopaminergic neurons. Importantly, the patient hiNPCs show different apoptotic marker expression. Thus, these hiNPCs, in addition to hiPSCs, are a favorable option to study PD pathology.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Stem Cells / Tissue Donors / In Vitro Techniques / Cell Line / Sendai virus / Induced Pluripotent Stem Cells / Dopaminergic Neurons / Karyotype / Fibroblasts Type of study: Prognostic study Limits: Humans Language: English Journal: International Journal of Stem Cells Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Stem Cells / Tissue Donors / In Vitro Techniques / Cell Line / Sendai virus / Induced Pluripotent Stem Cells / Dopaminergic Neurons / Karyotype / Fibroblasts Type of study: Prognostic study Limits: Humans Language: English Journal: International Journal of Stem Cells Year: 2019 Type: Article