Asiatic Acid Induces Apoptosis and Autophagy and Reduces MiR-17 and MiR-21 Expression in Pancreatic Cancer Cell Lines
Natural Product Sciences
;
: 298-303, 2019.
Article
in English
| WPRIM
| ID: wpr-786431
ABSTRACT
This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oncogenes
/
Pancreatic Neoplasms
/
Autophagy
/
Cell Line
/
Cell Survival
/
Blotting, Western
/
Apoptosis
/
Annexin A5
/
Sirolimus
/
MicroRNAs
Language:
English
Journal:
Natural Product Sciences
Year:
2019
Type:
Article
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