Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth / 대한소아치과학회지
Journal of Korean Academy of Pediatric Dentistry
;
(4): 85-92, 2019.
Article
in Korean
| WPRIM
| ID: wpr-787351
ABSTRACT
The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-Seal™ (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation RM > EN > EZ (p = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Stem Cells
/
Tooth, Deciduous
/
Calcium
/
Culture Media
/
Flow Cytometry
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Korean Academy of Pediatric Dentistry
Year:
2019
Type:
Article
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