Effects of Cyclic Nucleotide-Gated Channels in Vestibular Nuclear Neurons / 전남의대학술지
Chonnam Medical Journal
; : 155-159, 2011.
Article
in En
| WPRIM
| ID: wpr-788216
Responsible library:
WPRO
ABSTRACT
This study was designed to investigate the effects an 8-Br-cGMP on the neuronal activity of rat vestibular nuclear cells. Sprague-Dawley rats aged 14 to 16 days were decapitated under ether anesthesia. After treatment with pronase and thermolysin, the dissociated vestibular nuclear cells were transferred into a chamber on an inverted microscope. Spontaneous action potentials and potassium currents were recorded by standard patch-clamp techniques under current and voltage-clamp modes. Twelve vestibular nuclear cells revealed excitatory responses to 1-5 microM of 8-Br-cGMP, and 3 neurons did not respond to 8-Br-cGMP. Whole potassium currents of vestibular nuclear cells were decreased by 8-Br-cGMP (n=12). After calcium-dependent potassium currents were blocked by tetraethylammonium, the potassium currents were not decreased by 8-Br-cGMP. These experimental results suggest that 8-Br-cGMP changes the neuronal activity of vestibular nuclear cells by blocking the calcium-dependent potassium currents that underlie the afterhyperpolarization.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Potassium
/
Action Potentials
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Pronase
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Thermolysin
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Rats, Sprague-Dawley
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Patch-Clamp Techniques
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Tetraethylammonium
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Ether
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Anesthesia
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Neurons
Limits:
Animals
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Humans
Language:
En
Journal:
Chonnam Medical Journal
Year:
2011
Type:
Article