Your browser doesn't support javascript.
loading
RBC Enzyme Analysis / 임상소아혈액종양
Clinical Pediatric Hematology-Oncology ; : 8-12, 2013.
Article in Korean | WPRIM | ID: wpr-788489
ABSTRACT
Among ~20 RBC enzyme deficiencies causing hereditary hemolytic anemia (HRA), deficiencies involving three RBC enzymes such as glucose-6-phosphatase, pyruvate kinase and pyrimidine 5'-nucleodiase were known to be relatively common. The methods that have been used for RBC enzyme analysis are based on the kinetic spectrophotometry. This method, however, usually requires multiple step reactions and manual manipulations which are labor-intensive and time-consuming, and carry a greater risk of error due to their complexity. To solve this problem, we had successfully developed the multiplex enzyme analysis for galactose using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). We are now trying to adopt this method to other RBC enzymes associated with HRA. The devised method will allow simple, rapid, sensitive and reproducible quantification of RBC enzymes and should be helpful for the confirmatory diagnosis of HRA caused by RBC enzyme deficiencies.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyrimidines / Pyruvate Kinase / Spectrophotometry / Mass Spectrometry / Glucose-6-Phosphatase / Galactose / Anemia, Hemolytic, Congenital Type of study: Practice guideline Language: Korean Journal: Clinical Pediatric Hematology-Oncology Year: 2013 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyrimidines / Pyruvate Kinase / Spectrophotometry / Mass Spectrometry / Glucose-6-Phosphatase / Galactose / Anemia, Hemolytic, Congenital Type of study: Practice guideline Language: Korean Journal: Clinical Pediatric Hematology-Oncology Year: 2013 Type: Article