Effects of ligustrazine on autophagy-related proteins after spinal cord ischemia-reperfusion injury / 国际中医中药杂志

ABSTRACT

Objective To investigate the effect of ligustrazine on autophagy-related proteins Beclin 1,LC3 and P62 after spinal cord ischemia-reperfusion injury.Methods A total of 48 SD rats were randomly divided into sham operation group,model group,ligustrazine group and 3-MA group.The rats were intraperitoneally injected with ligustrazine injection 0.16 mg/kg in the Ligustrazine group,the rats were intraperitoneally injected with 3-methyladenine injection 0.015 mg/kg in the inhibitor group,and the rats were intraperitoneally injected with normal saline of equal volume in the sham operation group and model group.Spinal cord ischemia-reperfusion model was established in all groups except sham-operated group after administration.After molding behavioral scores were scored after 3 and 6 hours of ischemia,and the expression of Beclin 1,LC3 and P62 was detected by immunohis-tochemistry.Results After 3 and 6 hours,compared with the model group,the behavioral score (3 h2.33 ± 0.58 vs.0.67 ± 0.58,6 h3.33 ± 0.58 vs.1.33 ± 0.58) of the rats in ligustrazine group significantly increased (P＜0.05).Compared with the model group,the expression of Beclinl (3 h348.00×104± 0.27×104 vs.659.00×104± 0.11×104;6 h38.00×104± 0.19×104 vs.557.00×104± 0.26×104),LC3 (3 h357.00×104± 0.48×104 vs.686.00×104± 0.33×104'6 h334.00×104± 0.51×104 vs.673.00×104 ± 0.22×104),P62 (3 h357.00×104 ± 0.48×104 vs.830.00×104 ± 0.48×104;6 h315.00×104 ± 0.12× 104 vs.591.00× 104± 0.36× 104) in ligustrazine group were significantly decreased (P＜0.05).Conclusions The ligustrazine may regulate autophagy in two directions and protect nerve cells.