Determination of dexamethasone acetate in Kelaweiding liniment by HPLC / 药学实践杂志

ABSTRACT

Objective To establish the quantitative determination method of dexamethasone acetate in Kelaweiding lini‐ment by HPLC .Methods ColumnDiamonsil‐C18 (150 mm × 4 .6 mm ,5 μm) ,mobile phasemethanol‐water‐glacialaceticacid (80200 .5) ,flow rate1 .0 ml/min ,UV detection wavelength240 nm ,injection volume20 μl ,column temperature30 ℃ . Results The standard curve was linear within the range of 2 .00‐64 .00μg/ml ,linear equation was Y=0 .405 2X+0 .804 2(r=0 .999 9) .The average recovery was 99 .3% with RSD 0 .93% (n=9) .Conclusion This method was simple ,stable and could be used to assay dexamethasone acetate in Kelaweiding liniment .