HPLC method to assay the content of psoralen and angelicin in Zhuanggu granules / 药学实践杂志
Journal of Pharmaceutical Practice
;
(6): 441-442,458, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-790651
ABSTRACT
Objective To develop a HPLC method for psoralenand angelicin content assay in Zhuanggu granules .Meth-ods Agilent Zorbax C18 column(4 .6 mm × 250 mm ,5 μm) was used with methanol and water (45∶55) as mobile phase .The detection wavelength was set at 245 nm .The flow rate was 1 .0 ml/min and column temperature at 25 ℃ .The injection volume was 10 μl .Psoralenand angelicin were extracted with 70% ethanol under water bath .Results The linearity was obtained over 3 .75~40 μg/mlfor psoralenand angelicin .The RSDs were less than 2% .The average recovery was between 94% and 105% . Conclusion This simple and accurate HPLC method was suitable for the quality control of Zhuanggu granules .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Pharmaceutical Practice
Year:
2016
Type:
Article
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