Preparation of the etoposide chitosan micelle and the effect of chitosan on etoposide intestinal absorption / 药学实践杂志

ABSTRACT

Objective To prepare the etoposide chitosan micelle, and investigate the effect of chitosan on etoposide intestinal absorption.Methods The etoposide chitosan micelle was prepared by dialysis.The drug encapsulation efficiency and drug loading efficiency were determined by HPLC.The intestine in rats was cannulated for in situ recirculation.The effects of different chitosan doses on the intestinal drug absorption and the effects of chitosan on the drug absorption at different intestinal locations were studied.Results The average particle size of etoposide chitosan micelle was 139.5 nm.The multi-dispersion coefficient was 0.569.The standard curve of etoposide was A =8 436.8 C-4963.8,r=1.0000.The intra-and inter-day precision values meetthe requirement.The drug encapsulation efficiency was (47.3±2.84)% and drug loading efficiency was (1.10±1.27)%.With the increase of the chitosan concentration, the absorption capacity of the unit area in the whole intestine was increased in different degrees.Chitosan exhibits its effects on etoposide absorptionat different intestinal sections in the following order ileum>jejunum>duodenum.Conclusion Chitosan promoted etoposide absorption induodenum, jejunum and ileum, especially in jejunum and ileum.