Research on the determination of total flavonoids in compound Yinchen mixture / 药学实践杂志
Journal of Pharmaceutical Practice
;
(6): 547-550, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-790815
ABSTRACT
Objective To establish a method for the determination of the total flavonoids content in compound Yinchen mixture by UV spectrophotometry .Methods Using rutin as comparison ,three coloration methods were studied to find the op-timal assay method .Results The sample was detected at 508 nm wavelength by NaNO2-Al(NO3 )3-NaOH reaction with rutin as reference .The rutin content had a liner relationship in the range of 0 .0125-0 .0626 g/L (n=9 ,r=0 .9999) ,and the aver-age recovery rate was 99 .49% with RSD of 0 .84% .Conclusion The NaNO2-Al(NO3 )3-NaOH coloration method is proved to be simple ,quick ,stable and reliable for the determination of total flavonoids in compound Yinchen mixture .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Pharmaceutical Practice
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS