Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 126-130, 2018.
Article
in Zh
| WPRIM
| ID: wpr-790849
Responsible library:
WPRO
ABSTRACT
Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions.Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA.Cell proliferations were detected by CCK-8 experiment.RT-PCR test was used to detect the expression of onco-genes Akt2 and K-ras.Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pan-creatic cancer cells.EGFR protein was increased by the interference of TRPV 1 expression.The proliferation rate of pancreatic cancer cells was decreased and Akt2,K-ras were significantly inhibited by TRPV1 over-expressing.Conclusion The expres-sion of TRPV1 in pancreatic cancer cells regulated the EGFR protein content.The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing.
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Index:
WPRIM
Language:
Zh
Journal:
Journal of Pharmaceutical Practice
Year:
2018
Type:
Article