A cell model for high-throughput screening lead compounds targeting HIF-1α for atherosclerosis treatment / 药学实践杂志
Journal of Pharmaceutical Practice
;
(6): 27-31, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-790891
ABSTRACT
Objective To establish a high-throughput in-vitro screening cell model for anti-atherosclerosis leading compounds.Methods Hypoxia response element (HRE) was cloned into a luciferase reporter vector, pGL3-Enhancer, to construct pGL3-HIF-1α-HRE.The THP-1human monocyte cell line was infected with the pGL3-HIF-1α-HRE and a stable cell line, THP-1-HIF-1α-HRE, was screened.Results Real-time PCR assay showed that HIF-1αexpression and luciferase activity in THP-1-HIF-1α-HRE cells was effectively upregulated by hypoxia.The increase of HIF-1αexpression and luciferase activity induced by hypoxia was significantly inhibited by lovastatin or curcumin.Conclusion THP1-HIF-1α-HRE, an in-vitro cell model for high-throughput screening lead compounds for anti-atherosclerosis (AS) was successfully established.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Language:
Chinese
Journal:
Journal of Pharmaceutical Practice
Year:
2019
Type:
Article
Similar
MEDLINE
...
LILACS
LIS